Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)

DNA Polymerases (ultra pure)

DNA Polymerases synthesize DNA from nucleotides. DNA polymerase is required for DNA replication, but also essential for many other activities in the cell, including genetic recombination, DNA repair, and reverse transcription. In molecular biology labs, DNA polymerases are used for applications such as cloning, PCR, and DNA sequencing.

Each DNA polymerase has special characteristics, making it useful for specific applications. Knowing these characteristics enables you to pick the right ultra pure DNA Polymerase for your application.

DNA Polymerase Selection Chart

Product Number	Product Description	Notable Characteristics	Application Suitability
KEM0026	Klenow (3’ → 5’ exo)	Deficient in both proofreading and nick translation nuclease activities Moderate strand displacement activity	A-tailing for NGS Strand displacement amplification DNA labeling
KEM0027	Klenow (3’ → 5’ exo)
KEM0028	phi29 DNA Polymerase (High concentration)	Highly processive Proofreading activity (3’ → 5’) Powerful strand displacement activity	Whole Genome Amplification
KEM0029	phi29 DNA Polymerase (Low concentration)
KEM0030	DNA Polymerase I	Exhibits 3’ → 5’ synthesis activity Exonuclease activities (both 3’ → 5’ and 5’ → 3’)	Second strand cDNA synthesis Nick-translation DNA labeling
KEM0031	Klenow Fragment	Exhibits synthesis and proofreading (3’ → 5’) nuclease activities Moderate strand displacement activity	DNA blunting by fill-in of 5’ overhang Second strand cDNA synthesis Sequencing Site-directed mutagenesis
KEM0032	Terminal deoxynucleotidyl Transferase (TdT)	Template-independent Catalyzes addition of deoxynucleotides to 3’OH of ss- or ds-DNA	Homopolymeric tailing to the 3’OH TUNEL assay 5’ RACE Labeling of 3’ end
KEM0033	T4 DNA Polymerase	Catalyzes extension of a primed DNA template (5’ → 3’ direction) Powerful 3’ → 5’ exonuclease activity Lacks inherent 5’ → 3’ exonuclease or strand displacement activity	DNA end-repair by fill-in of 5’ overhang Removal of 3’ overhang
KEM0034	Mako DNA Polymerase (3’ → 5’ exo)	Catalyzes extension of a primed DNA template (5’ → 3’ direction)	Protocols requiring no 3’ → 5’ or 5’ → 3’ exonuclease activity Applications requiring no strand displacement activity
KEM0035	Manta 1.0 DNA Polymerase (High concentration)	Thermophilic enzyme Deficient in both proofreading and nick translation nuclease activities	Applications requiring an enzyme with thermo-stable, strong strand replacement activity Applications requiring a Bst I large fragment equivalent SDA assy LAMP assay NEAR assay
KEM0036	Manta 1.0 DNA Polymerase (Low concentration)

DNA Ligases (ultra pure)

DNA Ligases form phosphodiester bonds between DNA strands, joining them together. DNA Ligase is responsible for repairing breaks in single and double stranded DNA. In molecular biology labs, DNA Ligase is commonly used for molecular cloning applications.

Each DNA Ligase has special characteristics, making it useful for specific applications. Knowing these characteristics enables you to pick the right ultra pure DNA Ligase for your application.

DNA Ligase Selection Chart

Product Description	T4 DNA Ligase	T4 DNA Ligase (Rapid)	T3 DNA Ligase	T7 DNA Ligase	E. coli DNA Ligase
Products No.	KEM0020	KEM0019	KEM0017	KEM0018	KEM0025
Units	150,000	240,000	900,000	900,000	2,500
Concentration	120,000 U/mL	600,000 U/mL	3,000,000 U/mL	3,000,000 U/mL	40,000 U/mL
Cofactor Required	ATP	ATP	ATP	ATP	NAD
Recommended for Cloning?	✓	✓
Recommended for cohesive ends	✓	✓	✓	✓	✓
Recommended for blunt ends	✓	✓	✓ (less efficient)	✓ (less efficient)	✓ (less efficient)
Recommended for nicks in dsDNA	✓	✓	✓	✓	✓
Recommended for joining of RNA & DNA hybrids	✓	✓
Application Suitability	Restriction cloning TA cloning Adapter ligation	NGS library construction Restriction cloning TA cloning Adapter ligation	Applications requiring good activity under high ionic strength (1M NaCl)	TALE Applications requiring high cohesive end ligation efficiency	cDNA cloning by replacement synthesis

Nucleic Acid Modifying Enzymes (ultra pure)

Many different enzymes exist that are able to modify DNA, RNA, and RNA:DNA hybrids. In molecular biology labs, these modifying enzymes are commonly used for molecular cloning and sequencing applications.

Each enzyme has special characteristics, making it useful for specific applications. Knowing these characteristics enables you to pick the right ultra pure Nucleic Acid Modifying Enzyme for your application.

Nucleic Acid Modifying Enzyme Selection Chart

Product No.	Product Description	Notable Characteristics	Application Suitability
KEM0006	T4 Polynucleotide Kinase	Catalyzes the transfer and exchange of ATP to 5’OH of ss- or ds-DNA and RNA	End-labeling DNA or RNA for probes End-labeling DNA or RNA for sequencing Addition of 5’ phosphates to oligonucleotides for subsequent ligation Removal of 3’ phosphoryl groups
KEM0001	Uracil DNA Glycosylase (UDG)	Catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar (leaving an abasic site)	Removal of uracil from DNA Control of carry-over contamination in PCR
KEM0002	Thermolabile Uracil DNA Glycosylase (UDG)	Catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar (leaving an abasic site) Completely inactivated in 1x reaction buffer at 50°C for 10 minutes.
KEM0004	Exonuclease III	Digests one strand of the dsDNA at a time Digests the RNA strand of RNA:DNA hybrids	Removal of nucleotide(s) from duplex DNA in the 3’ → 5’ direction
KEM0005	Lambda Exonuclease	Highly processive 5’ → 3’ double-stranded exonuclease Degrades one strand of the duplex	Highly processive 5’ → 3’ exonuclease Applications requiring activity on blunt and 5’ recessed ends
KEM0009	End-Repair Mix (High Concentration)	Mix of T4 DNA Polymerase and T4 Polynucleotide Kinase Converts to blunt-ended DNA	Converting DNA containing damaged or incompatible 5’ and/or 3’ protruding ends to 5’ phosphoryleted, blunt-ended DNA
KEM0010	End-Repair Mix (Low Concentration)
KEM0011	10x Uracil Cleavage System	Generates a single nucleotide gap at uracil residues	Excising uracil residues from DNA
KEM0014	Poly(A) Polymerase	Catalyzes the addition of AMP (from ATP) to the RNA 3’OH	Labeling of RNA with ATP or cordycepin Poly(A) tailing of RNA for cloning or affinity purification Translation of RNA transferred into eukaryotic cells
KEM0015	RNase H	Degrades the RNA strand of RNA:DNA hybrids	Removal of mRNA during second-strand cDNA synthesis Cleavage of RNA from RNA:DNA hybrids

RNA Ligases (ultra pure)

RNA Ligases form phosphodiester bonds between RNA strands, joining them together. In molecular biology labs, RNA Ligase is commonly used for mutagenesis of RNA, and end-labeling applications.

Each RNA Ligase has special characteristics, making it useful for specific applications. Knowing these characteristics enables you to pick the right ultra pure RNA Ligase for your application.

Product Description	T4 RNA Ligase I	T4 RNA Ligase 2	T4 RNA Ligase 2 (truncated)
Product No.	KEM0021	KEM0024	KEM0023
Units	10,000	4,500	500
Concentration	20,000 U/mL	30,000 U/mL	5,000 U/mL
Cofactor Required	ATP	ATP	None
Recommended for nicks in dsDNA?		✓
Recommended for joining of RNA:DNA hybrids?		✓
Recommended for labeling of RNA 3’ termini?	✓		✓
Recommended for joining ssDNA?	✓
Recommended for joining ssRNA?	✓