LC/MS Analysis of β-Lactoglobulin Tryptic Digest on a Discovery^® BIO Wide Pore C18 Capillary Column

column

Discovery BIO Wide Pore C18, 15 cm × 0.5 mm I.D., 5 μm particles (65519-U)

mobile phase

[A] 0.1% TFA in water; [B] 0.1% TFA in acetonitrile

gradient

5-40% B in 70 min

flow rate

14 μL/min

column temp.

30 °C

detector

+ESI mode

injection

500 pmol (5μL) β-Lactoglobulin tryptic digest in 50 mM ammonium bicarbonate