HPLC Analysis of Plasmids on TSKgel® DNA-NPR
Materials
Analytical column
Product No.
Description
Pricing
TSKgel® DNA-NPR HPLC Column
phase DEAE (diethylaminoethyl), L × I.D. 7.5 cm × 4.6 mm, 2.5 μm particle sizeGuard column
Product No.
Description
Pricing
TSKgel® DEAE-NPR Guard Column
phase DEAE (diethylaminoethyl), L × I.D. 0.5 cm × 4.6 mm, 2.5 μm particle sizemobile phase component
Product No.
Description
Pricing
Sodium chloride
for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)CONDITIONS
column
TSKgel DNA-NPR, 7.5 cm x 4.6 mm, 2.5 μm particles (818249)
mobile phase
[A] 0.02 M Tris, pH 9.0; [B] 0.02 M Tris, 1.0 M NaCl, pH 9.0
gradient
50 to 60% B in 10 column volumes
flow rate
1 mL/min
detector
UV, 260 nm
sample
PUC19 plasmid
Description
Other Notes
Data provided by Tosoh Bioscience LLC.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation