HPLC Analysis of Finasteride Tablets on Chromolith^® HighResolution RP-18 endcapped 50 x 4.6mm

sample preparation

Dissolve an accurately weighed quantity of USP Finasteride RS in Diluting solution, and dilute quantitatively, and stepwise if necessary, with Diluting solution to obtain a solution having a known concentration of about 100 μg per mL.

column

Chromolith HighResolution RP-18 endcapped, 50 x 4.6 mm (1.52021.0001)

mobile phase

[A] acetonitrle; [B] 2.5 mM phosphoric acid in Water; (40:60,:A:B)

flow rate

2.25 mL/min

pressure

1160 psi 80 Bar

column temp.

45 °C

detector

240 nm cell: 11 μL

injection

10 μL

sample

Finasteride RS 100 μg/mL (100 ppm) (Diluent: Acetonitrile and water 70:30 (v/v))