HPLC Analysis of Bisphenol A in Spiked Milk Powder on Chromolith^® High Resolution RP-18e

sample preparation

12.1 g of milik powder dissolved in 100 mL hot water. Spike100 ppb of BPA to the solution, Add 0.1% of HCl and 10% acetonitrile. Add Internal standard BPF 60 ppb. Centrifuged and filter the sample with Mellix simplecity filiter.

column

Chromolith^® High Resolution RP-18 encapped, 100 x 4.6 mm (1.52022.0001)

mobile phase

[A] acetonitrile [B] MilliQ water; (25:75, A:B)

flow rate

Flow rate gradient

pressure

1305 psi (90 bar)

column temp.

35 °C

detector

Hitachi Chromaster-5440 Fluorescence Detector, λex=226; λem=306 nm (cell: 13 μL)

injection

10 μL

sample

Bisphenol A & 60ppb Bisphenol F in Methanol(5.2mg/10ml)