Synthesis of Fe3O4 poly(styrene-glycidyl methacrylate) magnetic porous microspheres and application in the immobilization of Klebsiella sp. FD-3 to reduce Fe(III)EDTA in a NO(x) scrubbing solution.

Synthesis of Fe3O4 poly(styrene-glycidyl methacrylate) magnetic porous microspheres and application in the immobilization of Klebsiella sp. FD-3 to reduce Fe(III)EDTA in a NO(x) scrubbing solution.

Bioresource technology (2013-01-22)

Xiaoyan Wang, Zuoming Zhou, Guohua Jing

ABSTRACT

Magnetic poly(styrene-glycidyl methacrylate) porous microspheres (MPPM) with high magnetic contents were prepared by surfactant reverse micelles and emulsion polymerization of monomers, in which the well-dispersed Fe(3)O(4) nanoparticles were modified by polyethylene glycol (PEG) and oleic acid (OA) respectively. The characterizations showed that both of the OA-MPPM and the PEG-MPPM were ferromagnetic, however, the OA-MPPM was used to immobilize the bacteria for more advantages. Therefore, the effects of monomer ratio, surfactant, crosslinker and amount of Fe(3)O(4) on the structure, morphology and magnetic contents of the OA-MPPM were investigated. Then, the OA-MPPM was utilized to immobilize Klebsiella sp. FD-3, an iron-reducing bacterium for Fe(III)EDTA reduction applied in NO(x) removal. Compared with free bacteria, the immobilized FD-3 showed a better tolerance to the unbeneficial pH and temperature conditions.

MATERIALS

Product Number

Brand

Product Description

E6760

Sigma-Aldrich

Ethylenediaminetetraacetic acid ferric sodium salt, BioReagent, suitable for plant cell culture, crystalline

S6760

Sigma-Aldrich

Span^® 80, nonionic surfactant

09-1500

Sigma-Aldrich

Ethylenediaminetetraacetic acid iron(III) sodium salt, SAJ special grade, ≥95.0%

700320

Sigma-Aldrich

Iron oxide(II,III), magnetic nanoparticles solution, 5 nm avg. part. size, 5 mg/mL in toluene

700304

Sigma-Aldrich

Iron oxide(II,III), magnetic nanoparticles solution, 20 nm avg. part. size, 5 mg/mL in toluene

700312

Sigma-Aldrich

Iron oxide(II,III), magnetic nanoparticles solution, 10 nm avg. part. size, 5 mg/mL in toluene

725331

Sigma-Aldrich

Iron oxide(II,III), magnetic nanoparticles solution, 5 nm avg. part. size, 5 mg/mL in H₂O

725366

Sigma-Aldrich

Iron oxide(II,III), magnetic nanoparticles solution, 20 nm avg. part. size, 5 mg/mL in H₂O

725358

Sigma-Aldrich

Iron oxide(II,III), magnetic nanoparticles solution, 10 nm avg. part. size, 5 mg/mL in H₂O

85548

Supelco

Span^® 80, viscosity 1000-2000 mPa.s (20 °C)

09569

Supelco

Span^® 80, suitable for GC

518158

Sigma-Aldrich

Iron(II,III) oxide, 99.99% trace metals basis

310069

Sigma-Aldrich

Iron(II,III) oxide, powder, <5 μm, 95%

EDFS

Sigma-Aldrich

Ethylenediaminetetraacetic acid iron(III) sodium salt, powder

637106

Sigma-Aldrich

Iron(II,III) oxide, nanopowder, 50-100 nm particle size (SEM), 97% trace metals basis