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Very bright europium complexes that stain cellular mitochondria.

Chemical communications (Cambridge, England) (2013-01-22)
James W Walton, Adrien Bourdolle, Stephen J Butler, Marine Soulie, Martina Delbianco, Brian K McMahon, Robert Pal, Horst Puschmann, Jurriaan M Zwier, Laurent Lamarque, Olivier Maury, Chantal Andraud, David Parker
ABSTRACT

The synthesis, structure and photophysical properties of a series of highly emissive europium complexes is reported. Certain complexes enter mammalian cells by macropinocytosis and stain the mitochondria selectively, allowing observation of the Eu emission in cellulo by time-gated spectral imaging.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Wortmannin, Ready Made Solution, from Penicillium funiculosum, ≥95% (HPLC)
Sigma-Aldrich
Amiloride hydrochloride hydrate, ≥98% (HPLC), powder
Sigma-Aldrich
Wortmannin, from Penicillium funiculosum, ≥98% (HPLC and TLC)