- Building a kinetic model of trehalose biosynthesis in Saccharomyces cerevisiae.
Building a kinetic model of trehalose biosynthesis in Saccharomyces cerevisiae.
Methods in enzymology (2011-09-29)
Kieran Smallbone, Naglis Malys, Hanan L Messiha, Jill A Wishart, Evangelos Simeonidis
PMID21943906
ABSTRACT
In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast. The biology of the trehalose cycle is briefly reviewed and discussed.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast, Type X, lyophilized powder, ≥40 units/mg protein