G006195
HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2
application for HPLC
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technique(s)
HPLC: suitable
test parameters
column: SUPELCOSIL LC-NH2, 25 cm x 4.6 mm I.D., 5 μm particles (58338)
mobile phase: [A] water; [B] acetonitrile; (25:75, A:B)
flow rate: 1 mL/min
temp.: ambient
detector: refractive index (RI)
injection: 2 μL
sample: 10 mg/mL in water
suitability
application for HPLC
application(s)
food and beverages
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Analyte
Description
Fructose, United States Pharmacopeia (USP) Reference Standard
Dextrose, United States Pharmacopeia (USP) Reference Standard
Sucrose, suitable for microbiology, ACS reagent, ≥99.0%
Maltose solution, for molecular biology, BioReagent, ~20% in H2O
Lactose, pharmaceutical secondary standard; traceable to USP and PhEur
Standard
analytical column
Product No.
Description
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