SRP2125

RXRα, ligand binding domain, (200-462), GST tagged human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(s): FLJ00280, FLJ00318, FLJ16020, FLJ16733, MGC102720, NR2B1
• UNSPSC Code: 12352200
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥80% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~56.5 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 350 μg/mL
• color: clear colorless
• NCBI accession no.: NM_002957
• UniProt accession no.: P19793
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... RXRA(6256)

Description

Biochem/physiol Actions

Retinoid X receptor (RXR) serves as a promiscuous heterodimerization partner for many nuclear receptors through the identity box, a 40-amino acid subregion within the ligand binding domain (LBD). RXR partners include thyroid hormone receptors (TRs), retinoic acid receptors (RARs), peroxisome proliferator-activated receptor, several constitutive active orphan nuclear receptors (e.g. nuclear growth factor I-B), oxysterol receptors, and constitutive androstane receptors. RXRs also form homodimers to mediate the effects of 9-cis-retinoic acid (9-cRA). Depending on these protein-protein interactions, RXR-containing complexes have distinct ligand-dependent and constitutive functions. The LBD is functionally complex and mediates ligand binding, receptor homo- and heterodimerization, repression of transcription in the absence of ligand, and ligand-dependent activation of transcription. Hormone binding to the structurally conserved LBD of the RXR triggers a conformational change that principally affects the conserved C-terminal transactivation helix H12 involved in transcriptional activation. Coactivators directly recruited by liganded receptors include members of the steroid receptor coactivator/p160 family such as SRC-1, transcriptional intermediary factor 2/glucocorticoid receptor interacting protein 1, and RAC3/activator of thyroid and retinoic acid receptors/amplified in breast cancer 1.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable