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SAB2500503

Sigma-Aldrich

Anti-HAP1 antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-HAP2, Anti-HIP5, Anti-HLP, Anti-Huntingtin-associated protein 1, Anti-hHLP1

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About This Item

MDL number:
MFCD01633033
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

goat

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

indirect ELISA: suitable
western blot: suitable

UniProt accession no.

P54257

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HAP1(9001)

General description

The gene HAP1 (huntingtin-associated protein 1) encodes a nuclear enzyme that is mainly expressed in neurons. It is the human homolog of exonuclease III protein from E. coli. The gene is mapped to human chromosome 17q21.2-q21.3.

Immunogen

Peptide with sequence C-RYDFRYSEDREQ from the internal region of the protein sequence according to NP_003940.2; NP_817084.1.

Biochem/physiol Actions

The gene HAP1 (huntingtin-associated protein 1) is a bifunctional enzyme that functions as a DNA repair enzyme as well as in redox activation of transcription factors. It binds to the Huntington′s disease (HD) protein huntingtin in a polyglutamine length-dependent manner. It associates with huntingtin via the cytoskeletal proteins dynactin and pericentriolar autoantigen protein 1 forming coiled-coils. It plays a role in vesicle trafficking by facilitating interactions among cytoskeletal, vesicular and motor proteins. It is an apurinic/apyrimidinic (AP) site-specific DNA repair endonuclease that removes the AP sites produced naturally or by cytotoxic drugs and radiation. It also has RNAse H activity and controls the redox state of certain proto-oncogene products, such as the transcription factor c-Jun. The HAP1 protein is a candidate for pathology associated with HD.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied at 0.5 mg/mL in Tris saline with 0.02% sodium azide and 0.5% bovine serum albumin.

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A role for the human DNA repair enzyme HAP1 in cellular protection against DNA damaging agents and hypoxic stress.
Walker LJ
Nucleic Acids Research, 22, 4884-4889 (1994)
Assignment of the huntingtin (Hdh) gene, the huntingtin associated protein (Hap1) gene, and the huntingtin interacting protein (Hip1) gene to rat chromosomes 14, 10, and 12 by radiation hybrid mapping and fluorescent in situ hybridization.
Zimdahl H
Cytogenetics and Cell Genetics, 94, 101-104 (2001)
L J Walker et al.
Molecular and cellular biology, 13(9), 5370-5376 (1993-09-01)
The DNA binding activity of the c-jun proto-oncogene product is inhibited by oxidation of a specific cysteine residue (Cys-252) in the DNA binding domain. Jun protein inactivated by oxidation of this residue can be efficiently reactivated by a factor from
Huntingtin-associated protein 1 (HAP1) interacts with the p150Glued subunit of dynactin.
Engelender S
Human Molecular Genetics, 6, 2205-2212 (1997)

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