A211200

DMT-2′Fluoro-dA(bz) Phosphoramidite

• Synonym(s): DMT-2′Fluoro-dA(bz) Amidite
• Empirical Formula (Hill Notation): C₄₇H₅₁FN₇O₇P
• Molecular Weight: 875.92
• MDL number: MFCD12912392
• UNSPSC Code: 41116105
• PubChem Substance ID: 329770749
• NACRES: NA.51

Properties

• biological source: non-animal source (no BSE/TSE risk)
• product line: Proligo Reagents
• assay: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• form: powder
• technique(s): oligo synthesis: suitable
• impurities: ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤0.5% single Impurity (reversed phase HPLC); ≤3% residual Solvent content
• color: white to off-white
• λ: conforms (UV/VIS Identity)
• suitability: conforms to structure for H-NMR; conforms to structure for LC-MS
• nucleoside profile: base: deoxyadenosine; base protecting group: benzoyl; 2' protecting group: fluoro; 5' protecting group: DMT; deprotection: fast/standard
• storage temp.: −20°C
• SMILES string: CC(C)N(C(C)C)P(OCCC#N)O[C@H]([C@H](O1)COC(C2=CC=C(OC)C=C2)(C3=CC=C(OC)C=C3)C4=CC=CC=C4)[C@@H](F)[C@@H]1N5C6=NC=NC(NC(C7=CC=CC=C7)=O)=C6N=C5
• InChI: 1S/C47H51FN7O7P/c1-31(2)55(32(3)4)63(60-27-13-26-49)62-42-39(61-46(40(42)48)54-30-52-41-43(50-29-51-44(41)54)53-45(56)33-14-9-7-10-15-33)28-59-47(34-16-11-8-12-17-34,35-18-22-37(57-5)23-19-35)36-20-24-38(58-6)25-21-36/h7-12,14-25,29-32,39-40,42,46H,13,27-28H2,1-6H3,(H,50,51,53,56)/t39-,40-,42-,46-,63?/m1/s1
• InChI key: VCCMVPDSLHFCBB-MSIRFHFKSA-N

Description

General description

DMT-2′Fluoro-dA(bz) Phosphoramidite is a 2′Fluoro phosphoramidite that is used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.

Its key features include,

• Can be employed together with DNA or RNA phosphoramidites
• Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C
• Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
• Consistent lot-to-lot high purity and performance
• Manufactured under a certified ISO 9001 quality system

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable