T8787

Triton^™ X-100

for molecular biology

• Synonym(s): t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• CAS Number: 9036-19-5
• Beilstein/REAXYS Number: 2315025
• MDL number: MFCD00128254
• UNSPSC Code: 12161900
• PubChem Substance ID: 329826885
• NACRES: NA.21

Properties

• biological source: synthetic (organic)
• grade: for molecular biology
• description: non-ionic
• form: liquid
• mol wt: micellar avg mol wt 80,000; average mol wt 625
• aggregation number: 100-155
• technique(s): protein purification: suitable; protein quantification: suitable; western blot: suitable
• impurities: <1.00% water (Karl Fischer)
• pH: 9.7
• CMC: 0.2-0.9 mM (20-25°C)
• mp: 6 °C
• transition temp: cloud point 65 °C; pour point ~7 °C
• solubility: water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
• density: 1.06 g/mL at 25 °C (lit.)
• cation traces: Fe: <5 ppm; K: <0.05%; Na: <0.1%; heavy metals: <5 ppm
• HLB: 13.5
• application(s): hematology; histology
• foreign activity: DNase and RNase, none detected
• storage temp.: room temp
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton^™ X-100 has been used:

• In immunohistochemistry for staining the Flat-mount retinas
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
• To permeabilise cells during Immunofluorescent microscopic studies
• As a positive control in LDH assay to determine the cell membrane integrity
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
• For the preparation of outer membrane protein exctract
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells
• In the treatment of tissue sections for Immunofluorescence labeling

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

• Ideal for sensitive molecular biology research applications
• Tested for DNase and RNase
• Non-ionic nondenaturing detergent
• Superior wetting agent and emulsifier
• Highly versatile surfactant

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• pictograms: GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H315,H318,H410
• pcodes: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: 483.8 °F - closed cup
• flash_point_c: 251 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter