51457

Urea solution

BioUltra, ~8 M in H₂O

• Synonym(s): Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• Beilstein/REAXYS Number: 635724
• MDL number: MFCD00008022
• UNSPSC Code: 12352111
• PubChem Substance ID: 57651308
• NACRES: NA.25

Properties

• product line: BioUltra
• concentration: ~8 M in H₂O
• impurities: insoluble matter, passes filter test; ≤0.005% formaldehyde (HCHO); ≤0.05% biuret
• refractive index: n20/D 1.40
• pH: 8.0-10.0 (25 °C)
• anion traces: chloride (Cl^-): ≤5 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• cation traces: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.03; λ: 280 nm A_max: 0.02
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759) , CA2(760) , EPHX2(2053) ; mouse ... Ephx2(13850) ; rat ... Ppm1a(24666)

Description

Application

Urea solution has been used as a urea buffer for the denaturation of heavy chain and β2m inclusion body preparations. It has also been used as a component of 1x wash buffer to purify HIS-tagged microphthalmia-associated transcription factor (MITF).

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves