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09709

Sigma-Aldrich

Ammonium phosphate monobasic

BioUltra, ≥99.5% (T)

Synonym(s):

mono-Ammonium phosphate, prim-Ammonium phosphate, Ammonium dihydrogenphosphate

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About This Item

Linear Formula:
NH4H2PO4
CAS Number:
7722-76-1
Molecular Weight:
115.03
EC Number:
231-764-5
MDL number:
MFCD00003396
UNSPSC Code:
12161700
PubChem Substance ID:
329748806
NACRES:
NA.25

product line

BioUltra

Quality Level

200

assay

≥99.5% (T)

impurities

insoluble matter, passes filter test

pH

4.0-5.0 (25 °C, 0.1 M in H2O)

mp

190 °C (dec.) (lit.)

solubility

H2O: 0.1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.5 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤20 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.059
λ: 280 nm Amax: ≤0.050

SMILES string

N.OP(O)(O)=O

InChI

1S/H3N.H3O4P/c;1-5(2,3)4/h1H3;(H3,1,2,3,4)

InChI key

LFVGISIMTYGQHF-UHFFFAOYSA-N

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Application

Ammonium phosphate monobasic can be used to study optimization reagents, protein structural analysis, proteomics, x-ray crystallography and biological buffers. Ammonium phosphate monobasic has been used to develop a simple high-performance liquid chromatographic method for determining N1-alkylnicotinamides, including C1C5 alkyl derivatives, in urine. Ammonium phosphate monobasic has also been used to develop a high-performance liquid chromatographic assay coupled with UV detection (254 nm) for the determination of midazolam and two of its hydroxylated metabolites in human plasma.

Other Notes

Component of culture media e.g. DEV SIMMONS Citrate agar. Acetate agar acc. to Trabulsi and Ewing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL5122
BCCL9088
BCCL8867
BCCM1453
BCCK1036

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L.R. Trabulsi et al.
Publ. Hlth. Lab., 20, 137-137 (1962)
T Hirayama et al.
Analytical biochemistry, 147(1), 108-113 (1985-05-15)
A simple high-performance liquid chromatographic method has been developed for determining N1-alkylnicotinamides, including C1-C5 alkyl derivatives, in urine. N1-Alkylnicotinamides were reacted with acetophenone in strong alkali medium at 0 degrees C and then formic acid was added. The reaction mixture
María J Nozal et al.
Journal of pharmaceutical and biomedical analysis, 30(2), 285-291 (2002-08-23)
A high performance liquid chromatographic (HPLC) method for the assay of sumatriptan succinate residues in swabs collected from manufacturing equipment surfaces was developed and validated in order to control a cleaning procedure. The swabbing procedure using two cotton swabs moistened
Anelise Rodolfo Ferreira Pieralini et al.
The Journal of prosthetic dentistry, 108(1), 51-57 (2012-07-07)
Coatings of zirconite, Y(2)O(3) or ZrO(2) on wax patterns before investing in phosphate-bonded investments have been recommended to reduce the reaction layer in titanium castings, but they are not easily obtainable. Spinel-based investments are relatively stable with molten titanium and
Catarina Barbosa et al.
International journal of food microbiology, 160(2), 87-93 (2012-11-28)
Sulphur-containing amino acids, cysteine and methionine, are generally found in very low concentrations in grape-juice. The objective of this study was to identify the effects of methionine on aroma compounds formation. Nitrogen source effects on growth, fermentative behaviour and aroma
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