11467140001
Roche
DNA, MB-grade
from fish sperm
Synonym(s):
DNA
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41106310
Recommended Products
biological source
fish (sperm)
Quality Level
grade
Molecular Biology
form
solution (ready-to-use)
mol wt
40-1,000 kDa
packaging
pkg of 500 mg
manufacturer/tradename
Roche
concentration
100 μg/mL
color
light yellowopalescent
solubility
water: miscible
storage temp.
−20°C
General description
DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.
DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.
Application
The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Sequence
Chain Length 120 to 3,000 nucleotides
Physical form
Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0
Preparation Note
Working concentration: 100μg/ml
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
No data available
flash_point_c
No data available
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Yu Sasano et al.
Bio-protocol, 7(10), e2306-e2306 (2017-05-20)
Chromosome engineering is an important technology with applications in basic biology and biotechnology. Chromosome splitting technology called PCS (PCR-mediated Chromosome Splitting) has already been developed as a fundamental chromosome engineering technology in the budding yeast. However, the splitting efficiency of
Ryudo Ohbayashi et al.
Bio-protocol, 8(15), e2958-e2958 (2018-08-05)
Cyanobacteria are prokaryotic organisms that carry out oxygenic photosynthesis. The fresh water cyanobacterium Synechococcus elongatus PCC 7942 is a model organism for the study of photosynthesis and gene regulation, and for biotechnological applications. Besides several freshwater cyanobacteria, S. elongatus 7942
Jasmin van den Heuvel et al.
eLife, 10 (2021-07-29)
In humans and other holozoan organisms, the ribosomal protein eS30 is synthesized as a fusion protein with the ubiquitin-like protein FUBI. However, FUBI is not part of the mature 40S ribosomal subunit and cleaved off by an as-of-yet unidentified protease.
Dilip Kumar et al.
Journal of immunology (Baltimore, Md. : 1950), 182(2), 1011-1020 (2009-01-07)
The MAPKs ERK, JNK, and p38 control diverse aspects of the immune response, including regulation of cytotoxin biology in NK cells and CTL. The chemokine CCL5 is coreleased with the cytotoxins, perforin, the granzymes, and granulysin, during the lethal hit
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service