MABE1784
Anti-NCoA-2 Antibody, clone 3Ti-3C11
Synonym(s):
Class E basic helix-loop-helix protein 75 (bHLHe75), NCoA-2, SRC2, Transcriptional intermediary factor 2 (hTIF2)
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All Photos(2)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
3Ti-3C11, monoclonal
mol wt
calculated mol wt 159 kDa
observed mol wt ~85 kDa
purified by
using protein G
species reactivity
mouse, human
species reactivity (predicted by homology)
feline, horse
packaging
antibody small pack of 100
technique(s)
ChIP: suitable
ELISA: suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... NCOA2(10499)
Specificity
Clone 3Ti-3C11 mouse monoclonal antibody that detects NCoA-2. It targets an epitope within 246 amino acids from the internal part of the protein.
Immunogen
His-tagged, recombinant, human NCoA-2 protein fragment (aa 624-1287).
Application
Quality Control Testing
Evaluated by Western Blotting with His-tagged, recombinant fragment of Human NCoA-2.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.
Tested Applications
ELISA Analysis: A representative lot detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NCoA-2 in Chromatin Immunoprecipitation application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508; Ganti, K.P., et al. (2017). Proc Natl Acad Sci USA. 114(6):E951-E960).
Western Blotting Analysis: A representative lot detected NCoA-2 in Western Blotting application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with His-tagged, recombinant fragment of Human NCoA-2.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.
Tested Applications
ELISA Analysis: A representative lot detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NCoA-2 in Chromatin Immunoprecipitation application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508; Ganti, K.P., et al. (2017). Proc Natl Acad Sci USA. 114(6):E951-E960).
Western Blotting Analysis: A representative lot detected NCoA-2 in Western Blotting application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Nuclear receptor coactivator 2 (UniProt: Q15596; also known as NCoA-2, Transcriptional intermediary factor 2, hTIF2, Class E basic helix-loop-helix protein 75 (bHLHe75)) is encoded by the NCOA2 (also known as BHLHE75, SRC2, TIF2) gene (Gene ID: 10499) in human. Nuclear receptor coactivator 2 (NCoA-2) functions as a transcriptional coactivator in various cellular processes. It belongs to the p160/steroid receptor coactivator (SRC) family of proteins. NCoA-2 plays a crucial role in the regulation of gene expression by interacting with nuclear receptors and other transcription factors. It possesses multiple functional domains, including a nuclear receptor interaction domain (NRID), a histone acetyltransferase (HAT) domain, and several transcription activation domains (ADs). NCoA-2 acts as a bridge between transcription factors and the transcriptional machinery, facilitating the recruitment of coactivators and chromatin remodeling complexes to target gene promoters. This coactivator function enables NCoA-2 to modulate diverse biological processes such as development, metabolism, cell proliferation, and differentiation. Dysregulation of NCoA-2 has been implicated in various diseases, including cancer, where it can promote tumor growth and progression.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Reconstitution
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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