GF10
Anti-TGF-α (Ab-2) Mouse mAb (213-4.4)
liquid, clone 213-4.4, Calbiochem®
Synonym(s):
Anti-Transforming Growth Factor-α
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
213-4.4, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, rat, hamster, canine, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
General description
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3 x 63 Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~6 kDa TGF-α protein.
Recognizes the ~6 kDa TGF-α protein in A2058 cells or skin tissue.
This Anti-TGF-α (Ab-2) Mouse mAb (213-4.4) is validated for use in Frozen Sections, Immunocytochemistry, Immunoblotting Paraffin Sections for the detection of TGF-α (Ab-2).
Immunogen
Human
recombinant human TGF-α (~6 kDa mature form)
Application
Frozen Sections (1-5 µg/ml)
Immunocytochemistry (1-5 µg/ml)
Immunoblotting (not recommended)
Paraffin Sections (1-10 µg/ml, saponin pre-treatment required)
Immunocytochemistry (1-5 µg/ml)
Immunoblotting (not recommended)
Paraffin Sections (1-10 µg/ml, saponin pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analysis Note
Positive Control
A2058 cells or skin tissue
A2058 cells or skin tissue
Other Notes
Coffey, R.J., et al. 1987. Nature328, 817.
Derynck, R. 1986. J. Cell. Biochem.32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem.261, 14408.
Lee, D.C., et al. 1985. Molec. Cell. Biol.5, 3644.
Kimball, E.S., et al. 1984. Cancer Res.44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press p. 165.
Derynck, R. 1986. J. Cell. Biochem.32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem.261, 14408.
Lee, D.C., et al. 1985. Molec. Cell. Biol.5, 3644.
Kimball, E.S., et al. 1984. Cancer Res.44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press p. 165.
Does not cross-react with human or mouse EGF. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Richard D Bland et al.
American journal of physiology. Lung cellular and molecular physiology, 292(6), L1370-L1384 (2007-02-13)
Failed alveolar formation and excess, disordered elastin are key features of neonatal chronic lung disease (CLD). We previously found fewer alveoli and more elastin in lungs of preterm compared with term lambs that had mechanical ventilation (MV) with O(2)-rich gas
Maria Vittoria Arcidiacono et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 30(3), 423-433 (2014-10-09)
In secondary hyperparathyroidism (SHPT), enhanced parathyroid levels of transforming growth factor-α (TGFα) increase EGF receptor (EGFR) activation causing parathyroid hyperplasia, high parathyroid hormone (PTH) and also reductions in vitamin D receptor (VDR) that limit vitamin D suppression of SHPT. Since
Maria Vittoria Arcidiacono et al.
Journal of the American Society of Nephrology : JASN, 19(10), 1919-1928 (2008-06-27)
In secondary hyperparathyroidism, enhanced expression of TGF-alpha in the parathyroid leads to its own upregulation, generating a feed-forward loop for TGF-alpha activation of its receptor, EGFR receptor (EGFR), which promotes parathyroid hyperplasia. These studies examined the role of activator protein
Amrei Strehl et al.
The Journal of biological chemistry, 282(10), 7046-7055 (2007-01-11)
Protein kinase C (PKC) isoforms regulate many platelet responses in a still incompletely understood manner. Here we investigated the roles of PKC in the platelet reactions implicated in thrombus formation as follows: secretion aggregate formation and coagulation-stimulating activity, using inhibitors
Emmet McIntyre et al.
Breast cancer research and treatment, 122(1), 105-110 (2009-09-18)
The levels of expression of the four receptors and eleven ligands composing the epidermal growth factor family were measured using immunohistochemical staining in one hundred cases of breast cancer. All of the family were expressed to some degree in some
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