FCMAB217F
Milli-Mark® Anti-CD235a-FITC Antibody, clone JC159
clone JC159, Milli-Mark®, from mouse
Synonym(s):
Glycophorin-A, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44
Recommended Products
biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified antibody
clone
JC159, monoclonal
species reactivity
human
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
General description
Glycophorin A, CD235a, is a single-pass membrane sialoglycoprotein and carrier of the blood group specificities M and N. The gene is located on chromosome 4q28-q31 and share sequence homology with genes encoding glycophorin B and glycophorin E (van der Schoot, 2002). Glycophorin A is expressed by erythroid cells beginning on morphologically recognizable erythroid precursors, just after the CFU-E stage, to the mature erythrocyte. Once glycophorin A is maximally expressed, the amount in each erythroid cell remains constant and does not appear to change during further maturation (Loken, 1987).
Specificity
Antibody recognizes Human CD235a antigen.
Immunogen
Membrane extracts of hairy cell leukemia prepared from human spleen
Application
Detect CD235a using this Milli-Mark Anti-CD235a-FITC Antibody, clone JC159 validated for use in FC.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
Quality
Evaluated by flow cytometry using human lymphocytes
Target description
16 kDa (calculated)
Physical form
Protein A purified
Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Storage and Stability
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Human Lymphocytes
Human Lymphocytes
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Asma S Al-Amoodi et al.
iScience, 27(2), 108882-108882 (2024-02-07)
For almost two decades, clinicians have overlooked the diagnostic potential of CD34neg hematopoietic stem cells because of their limited homing capacity relative to CD34posHSCs when injected intravenously. This has contributed to the lack of appeal of using umbilical cord blood
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