ABC300

Anti-CIDEC Antibody/FSP27

0.5 mg/mL, from rabbit

• Synonym(s): Cell death activator CIDE-3, Cell death-inducing DFFA-like effector protein C, Fat-specific protein FSP27 homolog, CIDEC/FSP27
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• concentration: 0.5 mg/mL
• technique(s): immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_001186480
• UniProt accession no.: Q96AQ7
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CIDEC(63924)

Description

General description

Cell death-inducing DFFA-like effector protein C (CIDEC), also known as Fat-specific protein FSP27 homolog (FSP27) is a protein expressed primarily in adipose tissue that plays a role in lipid storage and lipid droplet function and metabolism. CIDEC/FSP27 is also thought to play a role in adipocyte apoptosis as over expression in preadipocytes induces apoptosis, however, in mature adipocytes CIDEC/FSP27 expression provides resistance to apoptotic stimuli. CIDE/FSP27 is also a transcription factor in white adipose tissue, where the protein acts as a coactivator with CEBPB protein to control the expression of many genes including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. CIDEC/FSP27 is localized both in the nucleus and cytoplasmic endoplasmic reticulum as well as in lipid droplets. CIDEC/FSP27 expression can also be found in other tissues other than adipose tissue including heart, brain, kidney and liver. CIDEC/FSP27 levels are regulated via the ubiquitin proteasomal degradation pathway, and the CIDEC/FSP27 level is also dependent upon triacylglycerol synthesis, fatty acid availability and lipid droplet formation need.

Immunogen

Epitope: CIDE-N domain

KLH-conjugated linear peptide corresponding to the CIDE-N domain of human CIDEC/FSP27.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CIDEC/FSP27 in human colorectal cancer and human ileum tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-CIDEC Antibody/FSP27 is validated for use in Western Blotting and Immunohistochemistry for the detection of CIDEC/FSP27.

Quality

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CIDEC/FSP27 in 10 µg of HCT116 cell lysate.

Target description

~30 kDa observed. The calculated molecular weight is 27 kDa, however CIDEC/FSP27 has been shown as a ~30 kDa band in some western blots. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable