AB1534
Anti-Brain Derived Neurotrophic Factor Antibody
serum, Chemicon®
Synonym(s):
brain-derived neurotrophic factor
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
suitability
not suitable for activity/function inhibition
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BDNF(627)
General description
Brain Derived Neurotrophic Factor (BDNF) is a secreted protein that promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer’s and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF exists as monomers or homodimers and binds to TRKB/NTRK2 receptors.
Specificity
Brain Derived Neurotrophic Factor (BDNF). By dot blot the antibody shows less than 0.1% cross reactivity with recombinant human NT3, NT4/5 or mouse NGF.
Immunogen
A 10 amino acid peptide corresponding to the amino-terminal of mouse Brain Derived Neurotrophic Factor (BDNF) coupled to ovalbumin.
Application
Immunohistochemistry:
1:1000-1:2000 dilution of a previous lot was used in immunohistochemistry (see suggested protocol).
ELISA:
1:1000-1:2000 dilution of a previous lot was used in ELISA.
Bioassay:
Does not inhibit BDNF biological activity.
Optimal working dilutions must be determined by end user.
1:1000-1:2000 dilution of a previous lot was used in immunohistochemistry (see suggested protocol).
ELISA:
1:1000-1:2000 dilution of a previous lot was used in ELISA.
Bioassay:
Does not inhibit BDNF biological activity.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
This Anti-Brain Derived Neurotrophic Factor Antibody is validated for use in ELISA, IH, WB for the detection of Brain Derived Neurotrophic Factor.
Quality
Evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected BDNF on 10 µg of mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected BDNF on 10 µg of mouse brain lysates.
Target description
18 kDa, with a homodimer at ~27-30 kDa
Physical form
Rabbit polyclonal antiserum in buffer containing no preservatives. Lyophilized. Reconstitute with 50 μL of sterile distilled water.
Unpurified
Storage and Stability
Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Brain tissue
Brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Rodrigo A P Martins et al.
Journal of neurochemistry, 95(1), 244-253 (2005-09-27)
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CNS neuroscience & therapeutics, 19(7), 484-493 (2013-03-29)
The intravenous anesthetic propofol caused episodic memory impairments in human. We hypothesized propofol caused episodic-like spatial memory retention but not acquisition impairments in rats and rescuing cAMP response element-binding protein (CREB) signaling using selective type IV phosphodiesterase (PDEIV) inhibitor rolipram
André Luis Bombeiro et al.
The European journal of neuroscience, 48(5), 2152-2164 (2018-08-14)
Peripheral nerve injuries severely impair patients' quality of life as full recovery is seldom achieved. Upon axonal disruption, the distal nerve stump undergoes fragmentation, and myelin breaks down; the subsequent regeneration progression is dependent on cell debris removal. In addition
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Molecular pain, 15, 1744806919826789-1744806919826789 (2019-01-12)
Accumulating evidence shows that inhibition of glycogen synthase kinase-3beta (GSK-3β) ameliorates cognitive impairments caused by a diverse array of diseases. Our previous work showed that spared nerve injury (SNI) that induces neuropathic pain causes short-term memory deficits. Here, we reported
Qi Wang et al.
Brain stimulation, 16(5), 1401-1411 (2023-09-14)
Rett syndrome (RTT), caused by mutations in the X-linked gene encoding methyl-CpG binding protein 2 (MeCP2), severely impairs learning and memory. We previously showed that forniceal deep brain stimulation (DBS) stimulates hippocampal neurogenesis with concomitant improvements in hippocampal-dependent learning and
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