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Key Documents

506165

Sigma-Aldrich

p53, His•Tag®, Human, Recombinant, E. coli

Synonym(s):

Tumor Protein p53

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About This Item

UNSPSC Code:
12352203

assay

≥80% (SDS-PAGE)

Quality Level

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

foreign activity

Proteases, none detected

shipped in

wet ice

storage temp.

−70°C

General description

This product has been discontinued.



Recombinant, human, wild-type p53 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. p53, a tumor suppressor, blocks transformation and inhibits tumor cell growth. Numerous mutations in the p53 gene have been characterized in human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis. The purified protein is useful in DNA binding assays and as a protein kinase substrate.

Recombinant, human, wild-type p53 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. p53, a tumor suppressor, blocks transformation and inhibits tumor cell growth. Numerous mutations in the p53 gene have been characterized in human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis. The purified protein is useful in DNA binding assays and as a protein kinase substrate.

Biochem/physiol Actions

Typical DNA binding assays require 0.5 ng to 2 ng of p53 protein per assay.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM KCl, 30 mM sodium pyrophosphate, 5% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Liu, G., et al. 2003. J. Biol. Chem.278, 17557.
Zhang, L., et al. 2000. Cancer Res.60, 3655.
Fields, S., and Jang, S.K. 1990. Science249, 1046.
Michalovitz, D., et al. 1990. Cell62, 671.
Raycroft, L., et al. 1990. Science249, 1049.
Finlay, C.A., et al. 1989. Cell57, 1083.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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