492029

NRG1-β1/HRG1-β1 EGF Domain, Human, Recombinant

• UNSPSC Code: 12352203

Properties

• assay: ≥97% (SDS-PAGE)
• Quality Level: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤1.0 EU/μg protein Endotoxin
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Recombinant, human neuregulin 1-β1/heregulin 1-β1 epidermal growth factor-like domain (NRG1-β1/HRG1-β1 EGF Domain), consisting of amino acids 176-246 and expressed in E. coli. All NRG1 isoforms contain an EGF-like domain that is required for binding directly to the ErbB3 or ErbB4 receptor tyrosine kinases.

Biochem/physiol Actions

ED₅₀ = 0.06-0.3 ng/ml as measured in a serum-free proliferation assay using MCF-7 human breast adenocarcinoma cells.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm filtered acetonitrile, TFA, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Other Notes

Buonanno, A., and Fischbach, G.D. 2001. Curr. Opin. Neurobiol.11, 287.
Adlkofer, K. and Lai, C. 2000. Glia29, 104.
Garratt, A.N. et al. 2000. BioEssays22, 987.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3