420411

Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code.

• Synonym(s): Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested
• Empirical Formula (Hill Notation): C₁₈H₃₆N₄O₁₁ · xH₂SO₄
• CAS Number: 25389-94-0
• Molecular Weight: 484.50 (free base basis)
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white to off-white
• solubility: water: 10 mg/mL
• shipped in: ambient
• storage temp.: 10-30°C
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
• InChI key: OOYGSFOGFJDDHP-KMCOLRRFSA-N

Description

General description

Recommended working concentration: 100 µg/ml.

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity.

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 70S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity. Useful for the selection of kanamycin resistant plasmids in E. coli. Tested and found to be negative for cytotoxicity against HeLa cells. Contains more than 98% kanamycin A.

Contains >98% kanamycin A. Potency: ≥750 µg/mg

Biochem/physiol Actions

Primary Target
protein biosynthesis that acts on the 30S ribosome

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Reconstitution

Following reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Other Notes

Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
Cardon, G.H., et al. 1993. Plant J.3, 773.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties1, 690.
Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother.6, 516.
Umezawa, S., et al. 1968. J. Antibiot.21, 367.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• signalword: Danger
• hcodes: H360D
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 2