218785

Caspase-10, Human, Recombinant, E. coli

Caspase-10, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

• Synonym(s): Mch4, FLICE-2
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• assay: ≥95% (SDS-PAGE)
• form: liquid
• specific activity: ≥150,000 units/mg protein; ≥50,000 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

M.W. 11,000 and 18,000 (heterodimer).

Recombinant, human caspase-10 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4.

Physical form

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Kang, J.J., et al. 1999. J. Biol. Chem. 274, 3189.
Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301.
Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578.
Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2