14-936-M

NEK9, active

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected Sf21 cells
• Quality Level: 100
• technique(s): activity assay: suitable
• UniProt accession no.: Q8TD19
• Gene Information: human ... NEK9(91754)

Description

General description

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.

Packaging

Also available in 10μg size as catalog # 14-936 and in bulk as catalog # 14-936-K.

Also available in 250μg size as catalog # 14-936M and in bulk as catalog # 14-936-K.

Quality

Routinely evaluated by phosphorylation of MBP.

Physical properties

41 kDa

Physical form

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 20mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Storage and Stability

6 months at -70°C

Analysis Note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2