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W1754

Sigma-Aldrich

Water

PCR Reagent, suitable for PCR

Synonym(s):

H2O

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About This Item

Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
Beilstein/REAXYS Number:
2050024
EC Number:
231-791-2
MDL number:
MFCD00011332
UNSPSC Code:
12191602
PubChem Substance ID:
24900779
NACRES:
NA.25

product name

Water, PCR Reagent

grade

PCR Reagent

vapor density

<1 (vs air)

vapor pressure

3 mmHg

sterility

sterile-filtered

form

liquid

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, none detected
RNase, none detected

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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General description

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Application

Water has been used:

  • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
  • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
  • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
  • Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)

Suitability

Suitable for polymerase chain reaction (PCR)

Other Notes

Easily compare specifications for Water products with the Water specification table.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Certificates of Analysis (COA)

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Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
Fatty acid secretion by the white-rot fungus, Trametes versicolor
Guyu H, et al.
Journal of Industrial and Engineering Chemistry, 49(1) (2022)
If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana
Lodh N, et al.
Freshwater science, 34(3), 909-917 (2015)
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Protocols

SYBR® Green JumpStart™ Taq ReadyMix™

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Hot Start dNTP Protocol

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

Long and Accurate PCR Amplification of DNA (D8045)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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