P1016

Phenylalanine Ammonia-Lyase from Rhodotorula glutinis

Grade I, buffered aqueous glycerol solution, 0.8-2.0 units/mg protein

• Synonym(s): L-Phenylalanine ammonia-lyase, Phenylalanine Deaminase from Rhodotorula glutinis
• CAS Number: 9024-28-6
• Enzyme Commission number: 4.3.1.5 (BRENDA, IUBMB)
• MDL number: MFCD00131844
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: fungus (Rhodotorula glutinis)
• Quality Level: 200
• type: Grade I
• form: buffered aqueous glycerol solution
• specific activity: 0.8-2.0 units/mg protein
• shipped in: wet ice
• storage temp.: −20°C

Description

Application

Phenylalanine ammonia-lyase has been used in a study to assess the effect of heat treatment on lignification of postharvest bamboo shoots. It has also been used in a study to investigate the effect of light on gene expression and podophyllotoxin biosynthesis in Linum album cell culture.

Biochem/physiol Actions

Phenylalanine Ammonia-Lyase from Rhodotorula glutinis can be cleaved and inactivated by the proteases chymotrypsin, subtilisin, and trypsin.

Unit Definition

One unit will deaminate 1.0 μmole of L-phenylalanine to trans-cinnamate and NH₃ per min at pH 8.5 at 30 °C.

Physical form

Solution in 60% glycerol, 3 mM Tris-HCl, pH 7.5, containing up to 0.5 M (NH₄)₂SO₄

Analysis Note

Protein determined by biuret

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable