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Key Documents

MAK218

Sigma-Aldrich

Tace Inhibitor Screening Assay Kit

Sufficient for 100 Fluorometric tests

Synonym(s):

ADAM Metallopeptidase Domain 17, ADAM17 Inhibitor Screening Assay Kit, Tumor Necrosis Factor-α-converting enzyme Inhibitor Screening Kit

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About This Item

EC Number:
UNSPSC Code:
12352200
NACRES:
NA.84

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... ADAM17(6868)
mouse ... ADAM17(11491)
rat ... ADAM17(57027)

General description

Tumor necrosis factor-a converting enzyme (TACE; ADAM17) is a member of the ADAM (A Disintegrin And Metalloprotease) protein family implicated in cell-cell and cell-matrix interactions. TACE activates Notch signaling in the epidermal cells and has a critical function in the maintenance of barrier immunity. It is also involved in neurogenesis and muscle development.

Application

Tace Inhibitor Screening Assay Kit has been used in TACE (TNFa converting enzyme) activity assays.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the screening of inhibitors of TACE.

Principle

The TACE Inhibitor Screening Assay Kit is a rapid, simple and sensitive assay that is suitable for high throughput screening of TACE inhibitors. TACE hydrolyzes a FRET-tagged substrate to release a fluorescent product (λex = 318/ λem = 449 nm), proportional to the enzymatic activity present.

Kit Components Only

Product No.
Description

  • TACE Assay Buffer

  • TACE Substrate

  • TACE Enzyme

  • Inhibitor Control, 0.1 mM GM6001

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


Certificates of Analysis (COA)

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Joseph Dosch et al.
Oncotarget, 8(39), 65090-65099 (2017-10-17)
ADAM17 (a disintegrin and metalloproteinase 17)/TACE (TNFα converting enzyme) has emerged as a potential therapeutic target in colorectal cancer (CRC) and other cancers, due in part to its role in regulating various tumor cell surface proteins and growth factors and

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