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MAK120

Sigma-Aldrich

Urease Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Urea Amidohydrolase Activity Assay Kit

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1 KIT
$491.00

$491.00

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1 KIT
$491.00

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

$491.00

Usually ships in 2 weeks.
Request a Bulk Order

usage

sufficient for 100 colorimetric tests

application(s)

environmental

detection method

colorimetric

relevant disease(s)

gastrointestinal diseases

storage temp.

2-8°C

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General description

Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia.[1] Urease is found in bacteria, yeast, and certain higher plant species.[2] Urease activity is found in environmental and fecal samples.[3]

Application

Urease Activity Assay Kit has been used to measure urease activity.[4]

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of Urease activity in biological and environmental samples.

Principle

In this assay, urease catalyzes the hydrolysis of urea resulting in the production of ammonia. The ammonia is determined by the Berthelot method resulting in colorimetric product measure at 670 nm, proportionate to the urease activity present in the sample. One unit of urease is the amount of enzyme that catalyzes the formation of 1.0 μmole ammonia per minute at pH 7.0.

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Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

supp_hazards

EUH031

Storage Class

8B - Non-combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
120CF01A09
120CF01A09
120CE05A31
120CE04A29
120CE05A31

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Bhawna Uprety et al.
Pharmaceutics, 14(1) (2022-01-22)
Tumour cells maintain a local hypoxic and acidic microenvironment which plays a crucial role in cancer progression and drug resistance. Urease is a metallohydrolases that catalyses the hydrolysis of urea into ammonia and carbon dioxide, causing an abrupt increase of
Ureases I. Functional, catalytic and kinetic properties: A review
Barbara K.
Journal of Molecular Catalysis. B, Enzymatic, 59(1-3), 9-21 (2009)
Isolation, partial purification, characterization and inhibition of urease (EC 3.5. 1.5) enzyme from the Cajanus cajan seeds.
Sujoy B and Aggarwal A
Asian Journal of Bio Science, 7(2), 203-209 (2012)
Zeli Shen et al.
mSphere, 7(1), e0077221-e0077221 (2022-02-10)
In populations with similar prevalence of Helicobacter pylori infection, cancer risk can vary dramatically. Changes in composition or structure of bacterial communities in the stomach, either at the time of exposure or over the course of H. pylori infection, may
Malassezia furfur promoting growth of Staphylococcus epidermidis by increasing pH when cultured in a lipid-free environment.
Yang Han et al.
Chinese medical journal, 132(7), 873-876 (2019-03-22)
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