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MAK104

Sigma-Aldrich

Total Carbohydrate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

usage

sufficient for 100 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

storage temp.

2-8°C

General description

Carbohydrates are the most abundant biomolecules present in all living organisms. Carbohydrates have many functions, as structural components to the cell walls of bacteria and plants, or as energy storage in the form of starch and glycogen. Carbohydrates are also a major component of the human diet.[1][2] The Total Carbohydrate Assay Kit can be used for measuring carbohydrates in a variety of samples, including food and beverage products.

Application

Total Carbohydrate Assay Kit has been used in the colorimetric measurement of carbohydrates in biological wastewater treatment systems.[3]

Suitability

Suitable for measuring total carbohydrates in adherent or suspension cells, animal and plant tissues and food products, fruit juices & other beverages.

Principle

Total carbohydrate concentration is based on the phenol-sulfuric acid method in which polysaccharides are hydrolyzed and then converted to furfural or hydroxylfurfural. These compounds react with the Developer to generate a chromagen, which can be detected spectrophotometrically at 490 nm.

replaced by

Product No.
Description
Pricing

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

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Certificates of Analysis (COA)

Lot/Batch Number
8A12K06450
8J30K06450
8B23K06450
7J28K06450
7F10K06450

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Edgar Augusto Ortiz-Benítez et al.
Metallomics : integrated biometal science, 11(7), 1265-1276 (2019-06-08)
Streptococcus pneumoniae is a causal agent of otitis media, pneumonia, meningitis and severe cases of septicemia. This human pathogen infects elderly people and children with a high mortality rate of approximately one million deaths per year worldwide. Antibiotic-resistance of S.
Robert V S and Spencer W
Carbohydrates: The Essential Molecules of Life (2010)
Peio Elichiry-Ortiz et al.
Analytical and bioanalytical chemistry, 413(2), 519-531 (2020-11-07)
Sugar-enriched media are used to produce extracellular substances (ECS) by Lactobacillus plantarum WCSF1, with a focus on growing stages and carbon source substrates. Combination of size exclusion chromatography and ATR-FTIR spectroscopy provides physicochemical patterns of bulk ECS produced along culture
Nirmani N Wickramasinghe et al.
Scientific reports, 10(1), 16457-16457 (2020-10-07)
Psychrotrophic Pseudomonas species are the key spoilage bacteria of aerobically stored chilled meat. These organisms readily form biofilms on meat under refrigerated conditions leading to consumer rejection and associated economic losses. Limited information is available on the matrix composition of
Ebrahim Lari et al.
The Science of the total environment, 595, 594-600 (2017-04-12)
The increasing amount of stored oil sands process-affected water (OSPW), a primary by-product of oil sands mining, is an environmental concern. In the present study, we investigated the chronic effects of OSPW on growth, reproduction, and macronutrient content in Daphnia
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Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What are the recommended centrifuge settings and reagent mixing instructions for the MAK104 Total Carbohydrate Assay Kit? Is 2,000 x g sufficient for centrifugation?

    1 answer

    1. As per the guidelines:
      1. For sample preparation, the protocol suggests using a centrifuge speed of 12,000 RPM to ensure that all debris in the sample is properly sedimented before collecting the supernatant for the assay. While 2000 g is approximately equivalent to 4000 RPM and may be insufficient, customers can attempt this speed to see if a good pellet is obtained. However, a speed closer to 12,000 RPM is recommended. Typically, around 10,000 g for sample preparation centrifugation or a similar speed is suggested.

      2. The use of a horizontal or orbital shaker is suitable as long as the contents are not spilled. The goal is to gently mix the contents. Alternatively, a pipette can be used as long as it does not introduce air bubbles.

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  4. What are the detection sensitivity limit and the maximum concentration of carbohydrates that can be measured using the MAK104-1KT Total Carbohydrate Assay Kit?

    1 answer

    1. According to the guidelines provided, the MAK104-1KT Total Carbohydrate Assay Kit has a lower limit of detection of 4 micrograms and an upper limit of detection of 20 micrograms.

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  5. Hello, I am trying to determine how to dispose of the used assays after testing. The SDS does not provide any guidance.

    1 answer

    1. Please refer to section 13 of the SDS for information on disposal. Contact the facility and/or local EHS for further assistance.

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