35848

2′,7′-Dichlorofluorescein

BioReagent, suitable for fluorescence, ≥90% (T)

• Synonym(s): DCF, Dichlorofluorescein
• Empirical Formula (Hill Notation): C₂₀H₁₀Cl₂O₅
• CAS Number: 76-54-0
• Molecular Weight: 401.20
• Beilstein/REAXYS Number: 58009
• EC Number: 200-968-6
• MDL number: MFCD00005047
• UNSPSC Code: 12171500
• PubChem Substance ID: 57649439
• NACRES: NA.32

Properties

• product line: BioReagent
• assay: ≥90% (T)
• form: solid
• mp: 280 °C (dec.) (lit.)
• fluorescence: λ_ex 504 nm; λ_em 529 nm in 0.1 M Tris pH 8.0
• suitability: suitable for fluorescence
• SMILES string: Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
• InChI: 1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
• InChI key: VFNKZQNIXUFLBC-UHFFFAOYSA-N

Description

General description

2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Application

2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves