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  • Fatty-acid composition of rat brain lipids. Determined by support-coated open-tubular gas chromatography.

Fatty-acid composition of rat brain lipids. Determined by support-coated open-tubular gas chromatography.

Journal of chromatographic science (1976-01-01)
R K Pullarkat, H Reha
ABSTRACT

The nonhydroxy fatty acid composition of rat brain lipids (except gangliosides) was determined by support-coated open-tubular (SCOT) gas chromatography. Fatty acids of both odd and even chain lengths ranging from C14 to C26 were detected. Brain lipids contained 49% saturated, 29% monounsaturated, and 22% polyunsaturated fatty acids. Monoenoic fatty acids were mainly of the omega-9 and omega-7 series with minor amounts of omega-10 and amega-11 isomers. Dienes and trienes consisted of omega-6, amega-7, and omega-9 series. Tetraenes were of the omega-6 series. Small amounts of omega-6 and omega-3 pentaenes were detected. The most abundant polyunsaturated fatty acid was 22:6omega-3. The advantages of support-coated open-tubular columns over wall-coated open-tubular columns for the analysis of brain lipid fatty acids are discussed.

MATERIALS
Product Number
Brand
Product Description

Supelco
SCOT Capillary GC Column, phase BMEA
Supelco
SCOT Capillary GC Column, phase TCEP
Supelco
SCOT Capillary GC Column, phase Bentone 34/DNDP