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  • Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor.

Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor.

Nature (2020-04-01)
Jun Lan, Jiwan Ge, Jinfang Yu, Sisi Shan, Huan Zhou, Shilong Fan, Qi Zhang, Xuanling Shi, Qisheng Wang, Linqi Zhang, Xinquan Wang
ABSTRACT

A new and highly pathogenic coronavirus (severe acute respiratory syndrome coronavirus-2, SARS-CoV-2) caused an outbreak in Wuhan city, Hubei province, China, starting from December 2019 that quickly spread nationwide and to other countries around the world1-3. Here, to better understand the initial step of infection at an atomic level, we determined the crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2. The overall ACE2-binding mode of the SARS-CoV-2 RBD is nearly identical to that of the SARS-CoV RBD, which also uses ACE2 as the cell receptor4. Structural analysis identified residues in the SARS-CoV-2 RBD that are essential for ACE2 binding, the majority of which either are highly conserved or share similar side chain properties with those in the SARS-CoV RBD. Such similarity in structure and sequence strongly indicate convergent evolution between the SARS-CoV-2 and SARS-CoV RBDs for improved binding to ACE2, although SARS-CoV-2 does not cluster within SARS and SARS-related coronaviruses1-3,5. The epitopes of two SARS-CoV antibodies that target the RBD are also analysed for binding to the SARS-CoV-2 RBD, providing insights into the future identification of cross-reactive antibodies.

MATERIALS
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Product Description

Sigma-Aldrich
SARS-COV-2-Spike-RBD epitope (450-469), 95% (HPLC), lyophilized powder
Pricing and availability is not currently available.
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SARS-COV-2-Spike-RBD epitope (480-499), ≥95% (HPLC), lyophilized powder
Pricing and availability is not currently available.
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Anti-SARS-COV-2-Spike-RBD region Peroxidase conjugated antibody produced in rabbit
Pricing and availability is not currently available.
Sigma-Aldrich
SARS-COV-2-Spike-RBD epitope (370-394), ≥95% (HPLC), lyophilized powder
Pricing and availability is not currently available.