Anodic TiO2 nanotubular arrays with pre-synthesized hydroxyapatite--an effective approach to enhance the biocompatibility of titanium.

Anodic TiO2 nanotubular arrays with pre-synthesized hydroxyapatite--an effective approach to enhance the biocompatibility of titanium.

Journal of nanoscience and nanotechnology (2013-07-26)

Lu-Ning Wang, Long-Xiang Lin, Chang-Jian Lin, Chen Shen, Alyssa Shinbine, Jing-Li Luo

ABSTRACT

Electrochemically anodized TiO2 nanotubular arrays can provide large surface areas for biological species attachment. In order to further enhance the biocompatibility of Ti medical implants, we deposited a pre-synthesized hydroxyapatite inside and on the nanotubular arrays, and examined the biocompatibility of the anodized TiO2 nanotubular arrays with pre-synthesized hydroxyapatite by in vitro assessment in simulated body fluid, and in vitro cell culture. The results showed that the hydroxyapatite coating was able to be induced on TiO2 nanotubular arrays with pre-synthesized hydroxyapatite within 5 days while only a thin film composed of calcium phosphorous chemicals formed on as-formed TiO2 nanotubular arrays. The cell culture evaluation further proved the enhancement of cell attachment and proliferation on TiO2 nanotubular arrays with pre-synthesized hydroxyapatite as opposed to those without pre-synthesized hydroxyapatite. The present study proves that formation of TiO2 nanotubular arrays with pre-synthesized hydroxyapatite a promising method to enhance the biocompatibility of Ti implants.

MATERIALS

Product Number

Brand

Product Description

21223

Sigma-Aldrich

Hydroxyapatite, purum p.a., ≥90% (as Ca₃(PO₄)₂, KT)

H0252

Sigma-Aldrich

Hydroxyapatite, Type I, buffered aqueous suspension

04238

Sigma-Aldrich

Hydroxyapatite, puriss., meets analytical specification of Ph. Eur., BP, FCC, E341, ≥90% (calculated on glowed substance)

348848

Sigma-Aldrich

Titanium, foil, thickness 0.025 mm, 99.98% trace metals basis

267902

Sigma-Aldrich

Titanium, wire, diam. 0.81 mm, 99.7% trace metals basis

204757

Sigma-Aldrich

Titanium(IV) oxide, rutile, ≥99.98% trace metals basis

224227

Sigma-Aldrich

Titanium(IV) oxide, rutile, powder, <5 μm, ≥99.9% trace metals basis

369489

Sigma-Aldrich

Titanium, foil, thickness 2.0 mm, 99.7% trace metals basis

637262

Sigma-Aldrich

Titanium(IV) oxide, rutile, nanopowder, <100 nm particle size, 99.5% trace metals basis

204730

Sigma-Aldrich

Titanium(IV) oxide, rutile, 99.995% trace metals basis

IRMM531B

Titanium, IRMM^®, certified reference material, 0.5 mm foil

348791

Sigma-Aldrich

Titanium, foil, thickness 0.127 mm, 99.7% trace metals basis

635057

Sigma-Aldrich

Titanium(IV) oxide, rutile, <001>, (single crystal substrate), ≥99.9% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm

305812

Sigma-Aldrich

Titanium, 5-10 mm, ≥99.99% trace metals basis (purity exclusive of Na and K content)

634662

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanopowder, <100 nm particle size (BET), 99.5% trace metals basis

460400

Sigma-Aldrich

Titanium, wire, diam. 0.25 mm, 99.7% trace metals basis

IRMM531C

Titanium, IRMM^®, certified reference material, 0.5 mm wire

266035

Sigma-Aldrich

Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis

700347

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanoparticles, <150 nm particle size (volume distribution, DLS), dispersion, 40 wt. % in H₂O, 99.5% trace metals basis

637254

Sigma-Aldrich

Titanium(IV) oxide, anatase, nanopowder, <25 nm particle size, 99.7% trace metals basis

14027

Sigma-Aldrich

Titanium(IV) oxide, puriss., meets analytical specification of Ph. Eur., BP, USP, 99-100.5%

14021

Sigma-Aldrich

Titanium(IV) oxide, ReagentPlus^®, ≥99%

268496

Sigma-Aldrich

Titanium, powder, −100 mesh, 99.7% trace metals basis