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  • c-di-GMP can form remarkably stable G-quadruplexes at physiological conditions in the presence of some planar intercalators.

c-di-GMP can form remarkably stable G-quadruplexes at physiological conditions in the presence of some planar intercalators.

Chemical communications (Cambridge, England) (2011-03-15)
Shizuka Nakayama, Ilana Kelsey, Jingxin Wang, Herman O Sintim
ABSTRACT

The ubiquitous bacterial biofilm regulator, c-di-GMP can form G-quadruplexes at physiological conditions in the presence of some aromatic compounds, such as acriflavine and proflavine. The fluorescence of these compounds is quenched upon c-di-GMP binding and some of the formed c-di-GMP G-quadruplexes are stable even at 75 °C.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acriflavine, suitable for fluorescence, BioReagent, ≥90% (AT)
Sigma-Aldrich
Acriflavine, fluorescent label
Sigma-Aldrich
Acriflavine hydrochloride, fluorescent dye