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GE17-5087-02

Sigma-Aldrich

HiPrep 26/10 Desalting

Cytiva 17-5087-02, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

Synonym(s):

Desalting Column, HiPrep Desalting Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

polypropylene (PP)

mol wt

1000-5000 Da (globular proteins)

packaging

pack of 4

manufacturer/tradename

Cytiva 17-5087-02

storage condition

dry media

parameter

<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)

bed volume

53 mL

column L × I.D.

10 cm × 26 mm

matrix

cross-linked dextran
polymeric

matrix active group

dextran-epichlorohydrin phase

particle size

17-132 μm (dry)

cleaning

2-13

working range

2-13

separation technique

size exclusion (SEC)

General description

HiPrep 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

Features and Benefits

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

Suitability

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.

General instructions for affinity purification using HiTrap columns from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service