Skip to Content
Merck
All Photos(1)

Key Documents

GE17-0409-01

HiTrap® Chelating High Performance

Cytiva 17-0409-01, pack of 1 × 5 mL

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

iminodiacetic acid

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 17-0409-01

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-14

working range

3-13

General description

HiTrap® Chelating HP is prepacked with Chelating Sepharose High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Application

HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC System

Features and Benefits

  • For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.
  • Conveniently charge with your metal of choice.
  • Iminidoacetic acid coupled to Sepharose High Performance.
  • Purifying Proteins with exposed histidine groups via metal ion complex formation.
  • Simple operation with a syringe, pump or chromatographic system such as ÄKTA® design or FPLC System.
  • Packed with Chelating Sepharose High Performance
  • Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

See All

Protocols

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service