Skip to Content
Merck
All Photos(1)

Key Documents

GE17-0407-01

HiTrap® Heparin High Performance

Cytiva 17-0407-01, pack of 1 × 5 mL

Synonym(s):

Heparin Affinity Column

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

porcine heparin

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 17-0407-01

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

size

avg. part. size

34 μm

cleaning

5-10

working range

5-10

General description

HiTrap® Heparin HP columns are prepacked with Heparin Sepharose High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.

Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.

Heparin is coupled to the Sepharose High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.

Features and Benefits

  • Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.
  • Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.
  • Simple operation with a syringe, a pump or a chromatography system such as AKTA design
  • Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service