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A6686

Sigma-Aldrich

Agar

Bacteriological, microbiology tested, suitable for plant cell culture, granular

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
EC Number:
232-658-1
MDL number:
MFCD00081288
UNSPSC Code:
50405901
eCl@ss:
42040102
NACRES:
NA.28

biological source

algae (Rhodophyceae)

form

granular

technique(s)

cell culture | plant: suitable

mp

83-89  °C

transition temp

transition temp 32-39 °C

suitability

microbiology tested

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Agar, also known as agar-agar, is one of the biochemical agents most used in microbiology field. It is obtained from red algae Rhodophyceae, is the most abundantly used gelling agent in culture media. Agar is polysaccharide complex, composed of 70% agarose and 30% agaropectin.

Application

Agar has been used:
  • to culture Staphylococcus aureus for phage infection studies
  • as a supplement with NZCYM media for culturing Campylobacter coli strains for lysogeny screening
  • as a basic media component to improve in vitro ovule and embryo culture methods of cross Chitalpa hybrids to facilitate the rescue of triploids
  • in the culture media for Bdellovibrio spp. and Micavibrio spp. strains to check their susceptibility towards a variety of select agents

Other Notes

A purified agar from which the naturally occurring pigments, salts and miscellaneous matter has been reduced to a minimum.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C Carvalho et al.
Letters in applied microbiology, 50(2), 192-197 (2009-12-17)
Poultry meat is considered a major source of Campylobacter. This micro-aerobic bacterium is commonly responsible for foodborne illness. This work focuses on the isolation of Campylobacter coli lytic bacteriophages (phages) against target C. coli strains. A method involving the enrichment
Reproductive Behavior of Induced Allotetraploid ?Chitalpa and In Vitro Embryo Culture of Polyploid Progeny
Olsen R T, et al.,
J. Am. Soc. Hort. Sci., 131(6), 716-724 (2006)
Diana Gutiérrez et al.
Methods and protocols, 1(1) (2018-01-17)
The growing concern about multi-drug resistant pathogenic bacteria has led to a renewed interest in the study of bacteriophages as antimicrobials and as therapeutic agents against infectious diseases (phage therapy). Phages to be used for this purpose have to be
Riccardo Russo et al.
Microorganisms, 3(4), 903-912 (2015-01-01)
Select Agents are microorganisms and toxins considered to be exploitable as biological weapons. Although infections by many Select Agents can be treated by conventional antibiotics, the risk of an emerging or engineered drug resistant strain is of great concern. One
Arnaud Dechesne et al.
Applied and environmental microbiology, 74(16), 5195-5200 (2008-07-01)
Water is arguably the most important constituent of microbial microhabitats due to its control of physical and physiological processes critical to microbial activity. In natural environments, bacteria often live on unsaturated surfaces, in thin (micrometric) liquid films. Nevertheless, no experimental
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