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15663

Sigma-Aldrich

Boric acid

BioUltra, for molecular biology, ≥99.5% (T)

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About This Item

Linear Formula:
H3BO3
CAS Number:
10043-35-3
Molecular Weight:
61.83
EC Number:
233-139-2
MDL number:
MFCD00011337
UNSPSC Code:
12161700
eCl@ss:
38120104
PubChem Substance ID:
57647513
NACRES:
NA.25

grade

for molecular biology

vapor pressure

2.6 mmHg ( 20 °C)

product line

BioUltra

Assay

≥99.5% (T)

form

powder or crystals

technique(s)

DNA purification: suitable
RNA purification: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
<0.05% non-volatile substances in MeOH/HCl

loss

≤0.5% loss on drying, 20 °C (HV)

pH

3.6-4.4 (25 °C, saturated solution in H2O)

mp

160 °C (dec.) (lit.)

solubility

H2O: 1 M, clear, colorless (hot)

density

1.49 g/cm3 at 23 °C

anion traces

chloride (Cl-): ≤5 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤5 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.5 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

OB(O)O

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.015
λ: 280 nm Amax: 0.010

InChI

1S/BH3O3/c2-1(3)4/h2-4H

InChI key

KGBXLFKZBHKPEV-UHFFFAOYSA-N

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Application

Boric acid can be used to study molecular biology, DNA and RNA purification, biological buffers and molecular biology reagents. Boric acid has been used to test the toxic effects of boron on growth and antioxidant system parameters of maize (Zea mays L.) roots. Boric acid has also been used to study the effect of time period after boric acid injection on (10)B absorption in different regions of adult male rat′s brain.

Other Notes

Running buffer component with methylmercuric hydroxide gels; Buffer component in DNA digest analysis with capillary electrophoresis

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H360FD

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Paulus et al.
Electrophoresis, 14(1-2), 27-35 (1993-01-01)
DNA digest analysis with polymer-filled capillaries in capillary electrophoresis is described. The samples analyzed consisted of commercially available standards including a 100 base pair ladder with repeating units up to 2000 base pairs. Three DNA digests covered the most common
Electrophoresis in agarose and acrylamide gels.
R C Ogden et al.
Methods in enzymology, 152, 61-87 (1987-01-01)
Hye Ryeo Lee et al.
Journal of chromatography. A, 1346, 117-122 (2014-05-09)
In liquid phase microextraction, high enrichment factors can be obtained using an acceptor phase of small volume. By hanging an acceptor drop at the separation capillary tip, single drop microextraction (SDME) can be in-line coupled with capillary electrophoresis (CE). The
Albert Serrà et al.
ACS nano, 8(5), 4630-4639 (2014-05-03)
Electrodeposition from microemulsions using ionic liquids is revealed as a green method for synthesizing magnetic alloyed nanoparticles, avoiding the use of aggressive reducing agents. Microemulsions containing droplets of aqueous solution (electrolytic solution containing Pt(IV) and Co(II) ions) in an ionic
Sheng Tang et al.
Journal of chromatography. A, 1373, 31-39 (2014-12-02)
Layered double oxide hollow spheres (LDO-HSs) were synthesized and employed as a dissolvable sorbent in dispersive solid-phase extraction (DSPE) to extract eleven United States Environmental Protection Agency's priority phenols from aqueous samples. With their higher specific surface area, LDO-HSs showed
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