ELONGN-RO
Roche
Expand™ Long Range dNTPack
suitable for PCR, hotstart: no, Long Range PCR
Synonym(s):
long template pcr, longe range pcr, nucleotide mixes and sets, pcr enzymes
About This Item
usage
sufficient for ≤1000 reactions (04829069001)
sufficient for ≤200 reactions (04829042001)
sufficient for >50 reactions (04829034001)
Quality Level
feature
Long Range PCR
dNTPs included
hotstart: no
packaging
pkg of 3,500 U (04829069001 [5x700 U])
pkg of 175 U (04829034001)
pkg of 700 U (04829042001)
manufacturer/tradename
Roche
parameter
68 °C optimum reaction temp.
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
Related Categories
General description
Specificity
Application
- Genome mapping and sequencing
- Contig construction
- Characterization of cloned sequences in lambda phages or cosmids
- Cloning and analysis of eukaryotic genes
- Rapid identification and cloning of complete genes from genomic DNA using cDNA sequence as starting material
- Two-step RT-PCR
- PCR
- Large-fragment amplification
- RT-PCR
Features and Benefits
Amplify DNA fragments from 5 to 20kb from human genomic DNA, and 40kb from λDNA using supplied reaction buffers.
Use 3.75U for a standard 50μl PCR.
Each dNTPack contains 10mM additive-free sodium salt nucleotides as a ready-to-use mix.
- Amplify long templates
- Save time and resources.
- Use this kit for all long template applications.
- Cost-effective.
Packaging
Quality
Unit Definition
Preparation Note
Other Notes
Legal Information
Kit Components Only
- Expand Long Range Enzyme Mix
- Expand Long Range Buffer with 12.5 mM MgCl2 5x concentrated
- Expand Long Range Buffer without MgCl2 5x concentrated
- MgCl2 Stock Solution 25 mM
- DMSO 100%
- PCR Nucleotide Mix
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
203.0 °F
Flash Point(C)
95 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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