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Key Documents

SLM-241

Sigma-Aldrich

DMEM - high glucose

Complete medium, with ʟ-glutamine and sodium pyruvate and 10% FBS, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, DMEM with 10% FBS, Dulbecco′s Modified Eagle′s Medium - high glucose

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

product name

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS, contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS

Quality Level

300

form

liquid

technique(s)

cell culture | mammalian: suitable

General description

Dulbecco′s Modified Eagle (DMEM) Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient sterile solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells.

Application

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS has been used:
  • to culture human gingival fibroblasts
  • to culture mouse fibroblast cells
  • as an overlay medium for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plaque assay

Quality

pH= 7.2-7.5
Osmolality= 320-346
Endotoxin= ≤ 2EU/mL
Mycoplasma= Negative
Sterility Tested= Negatvie for Growth
Appearance= Orange/ Red Clear Liquid
Cell Line Tested= HT-22 Human Neuronal Cells

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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