371818

G-Protein βγ Binding Peptide, mSIRK

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes the α subunit dissociation without stimulating nucleotide exchange.

• Synonym(s): G-Protein βγ Binding Peptide, mSIRK, mSIRK, myr-SIRKALNILGYPDYD-OH
• Empirical Formula (Hill Notation): C₉₃H₁₅₀N₂₀O₂₅
• Molecular Weight: 1948.31
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes α subunit dissociation without stimulating nucleotide exchange. Reported to cause a rapid activation of ERK1/2 (EC₅₀ ~2.5-5 µM) in RASM (rat arterial smooth muscle) and Rat2 cells. Promotes the phosphorylation of JNK and p38 MAP kinase. Also increases phospholipase C activity and causes Ca²⁺ release from internal stores.

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes the α subunit dissociation without stimulating nucleotide exchange. Shown to cause a rapid activation of ERK1/2 (EC₅₀ ~ 2.5 - 5 µM) in rat arterial smooth muscle and Rat2 cells. Also promotes the phosphorylation of JNK and p38 MAP kinase, phospholipase C and causes Ca²⁺ release from internal stores. Negative control is also available (Cat. No. 371820).

Biochem/physiol Actions

Cell permeable: yes

EC50 ~ 2.5 - 5 µM in activation of ERK1/2 in rat arterial smooth muscle and Rat2 cells

Primary Target
N-myristoylated G-protein βγ selective binding

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Myr-N-Ser-Ile-Arg-Lys-Ala-Leu-Asn-Ile-Leu-Gly-Tyr-Pro-Asp-Tyr-Asp-OH

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable