Skip to Content
Merck
  • Engineering Styrene Monooxygenase for Biocatalysis: Reductase-Epoxidase Fusion Proteins.

Engineering Styrene Monooxygenase for Biocatalysis: Reductase-Epoxidase Fusion Proteins.

Applied biochemistry and biotechnology (2016-11-11)
Thomas Heine, Kathryn Tucker, Nonye Okonkwo, Berhanegebriel Assefa, Catleen Conrad, Anika Scholtissek, Michael Schlömann, George Gassner, Dirk Tischler
ABSTRACT

The enantioselective epoxidation of styrene and related compounds by two-component styrene monooxygenases (SMOs) has targeted these enzymes for development as biocatalysts. In the present work, we prepare genetically engineered fusion proteins that join the C-terminus of the epoxidase (StyA) to the N-terminus of the reductase (StyB) through a linker peptide and demonstrate their utility as biocatalysts in the synthesis of Tyrain purple and other indigoid dyes. A single-vector expression system offers a simplified platform for transformation and expansion of the catalytic function of styrene monooxygenases, and the resulting fusion proteins are self-regulated and couple efficiently NADH oxidation to styrene epoxidation. We find that the reductase domain proceeds through a sequential ternary-complex mechanism at low FAD concentration and a double-displacement mechanism at higher concentrations of FAD. Single-turnover studies indicate an observed rate constant for FAD-to-FAD hydride transfer of ~8 s

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
4-Methoxyindole, 99%
Sigma-Aldrich
5-Methoxyindole, 99%
Sigma-Aldrich
7-Azaindole, 98%
Sigma-Aldrich
1-Methylindole, ≥97%
Sigma-Aldrich
Methyl indole-5-carboxylate, 99%
Sigma-Aldrich
Methyl indole-6-carboxylate, 97%
Sigma-Aldrich
6-Bromoindole, 96%