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  • Dual regulatory actions of LncBMP4 on BMP4 promote chicken primordial germ cell formation.

Dual regulatory actions of LncBMP4 on BMP4 promote chicken primordial germ cell formation.

EMBO reports (2021-11-09)
Qisheng Zuo, Jin Jing, Jing Zhou, Yani Zhang, Wanhong Wei, Guohong Chen, Bichun Li
ABSTRACT

The unique characteristics of chicken primordial germ cells (PGCs) provide potential strategies for transgenic animal generation; however, insufficient PGC availability has limited their application. Regulation of bone morphogenic protein 4 (BMP4), a crucial factor for PGCs formation, may provide new strategies for PGC generation. We here identify a long noncoding RNA (lncRNA) that targets BMP4 (LncBMP4). LncBMP4 has similar functions as BMP4, in that it facilitates the formation and migration of PGCs. LncBMP4 promotes BMP4 expression by adsorbing the miRNA gga-mir-12211, thus reducing its inhibitory effect on BMP4 expression. In addition, the small peptide EPC5 encoded by LncBMP4 promotes the transcription of BMP4. The competing endogenous RNA (ceRNA) effect of LncBMP4 requires N6-methyladenosine (m6A) modification, in a dose-dependent manner, and high levels of m6A modification hinder EPC5 translation. Understanding the molecular mechanisms through which LncBMP4 promotes BMP4 expression during PGC formation may provide new avenues for efficient PGC generation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
ESGRO® Recombinant Mouse LIF Protein, ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml
Sigma-Aldrich
Stem Cell Factor Protein, Recombinant human, The Stem Cell Factor (SCF) Protein, Recombinant human, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis.
Sigma-Aldrich
Chicken Serum, USA origin, sterile-filtered, suitable for cell culture
Sigma-Aldrich
STM2457, ≥98% (HPLC)
Sigma-Aldrich
HumanKine® Thermostable bFGF,
Human Recombinant, Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.