Duolink® Proximity Ligation Assay
Duolink® Proximity Ligation Assays (PLA) enable the studying of endogenous Protein-Protein Interactions (PPI) and Post-Translational Modifications, providing a fluorescent signal for a single event that can be visualized by either microscopy or flow cytometry. To study Protein-Protein Interactions, Duolink® PLA technology uses a pair of antibodies labeled with oligonucleotides which when present within 40 nm undertake rolling circle amplification to generate a specific fluorescent signal after the addition of labeled probes.
Available in four different colors, for both flow cytometry and microscopy, Duolink® PLA allows for the visualization of multiple Protein-Protein Interactions per cell. Uncover the opportunities in your research with amplified performance and the benefits of Duolink® PLA technology:
- Visualize protein interactions – both stable and transient
- Visualize post-translational modifications
- Detect endogenous proteins – no overexpression or genetic manipulation
- High specificity – use of two antibodies/probes eliminates false positives
- No special equipment needed – standard immunofluorescence and flow cytometry-based methods available
Products
Duolink® PLA Products
- Duolink® PLA Starter Kits contains all the necessary reagents you need to perform a fluorescent experiment and analyze up to 30 samples.
- Duolink® Probemaker Kits enable the conjugation of a PLA oligo (PLUS or MINUS) to any antibody.
- Duolink® PLA Multicolor Kits allow for multiple PPI visualizations by microscopy.
- Duolink® flowPLA Detection Kits enables flow cytometry applications using four colors: green, orange, red, and far red.
- Duolink® PLA Brightfield Detection contains oligonucleotides labeled with horseradish peroxidase (HRP) allowing use with a light microscope.
Related Resources
- Application Note: An Introduction to Duolink® PLA
Duolink® PLA protein detection technology is based on in situ proximity ligation assay (PLA), and allows for the detection, visualization, and quantification of protein expression, protein-protein interactions, and post-translational modifications, in unmodified cells and tissue samples within a matter of hours.
- Duolink® Proximity Ligation Assay Instructional Video
This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.
- Application Note: How to Optimize the Duolink® Proximity Ligation Assay
To successfully execute a Duolink® PLA experiment, there are some necessary considerations to properly prepare for, setup, and execute the assay protocol.
- Brochure: Amplified Detection Duolink® Proximity Ligation Assay (PLA)
Rely on Duolink® PLA to amplify protein signals by 1000x, allowing you to visually detect proteins and their interactions, all with a straightforward protocol.
- Application Note: Duolink® PLA Probemaker Guide
This guide describes the use of the Duolink® In Situ Probemaker for the conjugation of PLA oligonucleotides (PLUS or MINUS) to any antibody for use in Duolink® PLA experiments.
- Flyer: Duolink® Flow Cytometry
Traditional flow cytometry has been limited in the ability to detect protein interactions and low abundant proteins events — until now.
- Duolink® PLA Fluorescence Protocol
This protocol describes the use of Duolink® PLA reagents for the immunofluorescent detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
- Duolink® PLA Brightfield Protocol
This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
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