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Merck
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69429

Millipore

EC RUG ChromoSelect Broth

NutriSelect® Plus, suitable for microbiology

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

form

powder

Quality Level

shelf life

limited shelf life, expiry date on the label

composition

buffers, 4.5 g/L
casein acid hydrolysate, 1.0 g/L
chromogenic mixture, 0.112 g/L
salts, 2.45 g/L
sodium chloride, 0.5 g/L
yeast extract, 2.0 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

7.0±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
veterinary
water monitoring

microbiology

storage temp.

2-8°C

suitability

Escherichia coli
coliforms

Application

For the detection of E. coli by the chromogenic and fluorogenic substrate resorufin-β-D-glucuronic acid methyl ester which has several advatages compared to MUG. E. coli appears as bright pink colony and is red fluorescence at 366 nm light source. In contrast to MUG, RUG is more specific and does not require fluorescent detection.

Preparation Note

Suspend 10.56 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE.Cool to 45-50°C. Dispense into sterile tubes or flasks as desired.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
RUG is a trademark of Biosynth

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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