55420

CLED Agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): BROLACIN Agar, Bromothymol-blue Lactose Cystine Agar, Cystine-Lactose-Electrolyte Deficient Agar
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; beef extract, 3 g/L ; bromo thymol blue, 0.02 g/L ; casein enzymic hydrolysate, 4 g/L ; L-cystine, 0.128 g/L ; lactose, 10 g/L ; peptic digest of animal tissue, 4 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | utilization test: suitable; microbiological culture: suitable
• final pH: 7.3±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; microbiology
• suitability: nonselective and differential for Enterococcus spp.; nonselective and differential for Escherichia coli; nonselective and differential for Klebsiella spp.; nonselective and differential for Lactobacillus spp.; nonselective and differential for Proteus spp.; nonselective and differential for Pseudomonas spp.; nonselective and differential for Salmonella spp.; nonselective and differential for Staphylococcus spp.; nonselective and differential for Streptococcus spp.; nonselective and differential for coliforms

Description

General description

CLED (cysteine-, lactose- and electrolyte-deficient) Agar is recommended for isolation, enumeration and identification of urinary pathogens.

CLED Agar is commonly used in bacteriological analysis.

Application

CLED Agar has been used as a non-selective media to perform enantiomeric profiling of quinolones and quinolones resistance gene qnrS in wastewater samples

Preparation Note

Suspend 36.15g in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves