07-1492

Anti-Calcineurin Aα Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): NFAT transcription complex cytosolic component, nuclear factor of activated T-cells, cytoplasmic,, calcineurin-dependent 1, nuclear factor of activated T-cells, cytosolic component 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, mouse, human
• species reactivity (predicted by homology): mammals (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_000935.1
• UniProt accession no.: Q08209
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PPP3CA(5530) ; mouse ... Ppp3Ca(19055) ; rat ... Ppp3Ca(24674)

Description

General description

Calcineurin is a serine/threonine protein phosphatase involved in the mediation of signal transduction in a wide variety of cellular responses. Calcineurin has been linked to Pmk1 MAP kinase and PI pathways, as well as to members of the small GTPase Rab/Ypt family and Type II myosin. Furthermore, calcineurin participates in the transduction of extracellular signals to the nucleus by dephosphorylation of members of the NFAT family of transcription factors. These linkages demonstrate calcineurin′s multifunctional capabilities in the regulation of calcium signaling. The Aα form of calcineurin is a major catalytic subunit gene isoform of calcineurin in most tissues that allows differentiation of Aα from the Aβ and Aγ isoforms.

Specificity

This antibody recognizes the Aα form of calcineurin.

Immunogen

KLH-conjugagted synthetic peptide corresponding to Calcineurin Aα.

Application

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

This Anti-Calcineurin Aα Antibody is validated for use in WB for the detection of Calcineurin Aα.

Quality

Evaluated by Western Blot in Mouse brain lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calcineurin on 10 µg of mouse brain lysate.

Target description

58 kDa

Linkage

Replaces: AB1696

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 40% gylerol. Contains no preservative.

Storage and Stability

Store at -20°C for up to 12 months in undiluted aliquots; at this temperature the solution will
remain unfrozen.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Mouse brain lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable